In this photosynthesis experiment, we are going to determine the effect of different light colors on the rate of photosynthesis. Photosynthesis is the phenomena in which solar energy is converted into useful bond energy. Different wavelengths (monochromatic light) have different effect on the photosynthesis. This experiment is performed to identify those changes.
Apparatus/Chemicals for Photosynthesis Experiment
Beaker, (Water Plant)fresh Hydrilla shoots, funnel, test tube, green red and blue papers, 0.1 % KHCO3 solution.
Procedure for Photosynthesis Experiment
- Take few healthy green shoots of Hydrilla and place them under a large funnel in a beaker containing 0.1% Potassium bicarbonate (KHCO3) solution. The level of the solution in the beaker should be a little above the stem of the funnel.
- Take a glass tube full of water and invert it on the stem of funnel.
- Keep this apparatus in sun light for some time.
- Note that the bubbles of the Oxygen are liberated from the hydrilla shoot and collected at the top of the tube.
- Note the time interval and the volume of the tube containing oxygen in it.
- Calculations can be made by finding out the volume of the space inside the tube. This will be equal to πr2l, where ‘r’ is the radius of the tube, and ‘l’ is the length of the space inside the tube.
- Cover the apparatus with green, red and then with blue papers one after the other.
- Repeat the procedure to calculate the production of oxygen under different colors. With every repetition, wait for 5 minutes before switching on to new calculation.
- Record your results in a table showing volume of oxygen evolved in the light of different colors.
Precautions for Photosynthesis Experiment
1) Do not disturb the apparatus during the experiment.
2) Add five minutes interval between the successive changes in color.
3) It is better to add fresh KHCO3 solution for every reading.
4) Use different colors of papers if not available then use color glass as a replacement.